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OBJECTIVE:To discuss the effect and mechanism of supplementing qi and nourishing yin(SQNY) decoction on intestinal microbiome of mice with Lewis lung carcinoma after treated with cisplatin. METHODS: The model of C57BL/6 mice with lung carcinoma xenograft was established by subcutaneous seeding of Lewis cells and randomly divided into 4 groups (n=16), including saline group (NC group), cisplatin group (DDP group), CAP group (CAP group) and SQNY decoction combined with cisplatin group (DDP+SQNY group). The tumor sizes, weights and intestinal microbiota were detected. The serum levels of IL-6, IL-10, and TNF-α were detected by ELISA. The dendritic cell (DC) and macrophage subtype in spleen of mice models were detected by flow cytometry. RESULTS: ①The mean tumor sizes of mice in DDP group, CAP group, DDP+SQNY groups were all smaller than NC group (P<0.05). And the mean tumor size and weight in DDP+SQNY group were less than the other three groups (P<0.05). The mean animal weights in NC or DDP+SQNY group were higher than those in DDP group and CAP group (P<0.05). ②The mean levels of serum IL-6, IL-10 and TNF-α in DDP+SQNY group were less than those in the other three groups (P<0.05). ③The mean microbiota richness in DDP+SQNY group were less than those in the other three groups (P<0.05). The proportion of fecal microbiota was significantly changed in DDP+SQNY group, including upregulation of Firmicutes phylum and Bifidobacterium Genus, and downregulation of Bacteroidetes phylum(P<0.05). The amount of DC and M1/M2 macrophage subtype in spleen of DDP+SQNY group mice models were more than those in the other three groups (P<0.05). CONCLUSION: It′s suggested that SQNY decoction effectively enhances the anticancer effect of cisplatin by increasing the immune function and changing the intestinal microbiota.
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<p><b>BACKGROUND</b>Diabetes has been associated with increased risk of fracture and impaired fracture healing. The aim of this study was to examine the influence of diabetes on perioperative complications, length of stay and ambulatory ability recovery in individuals with hip fracture, and to determine whether changes could be made to improve treatment outcome.</p><p><b>METHODS</b>The study included 707 hip fracture patients treated at Beijing Jishuitan Hospital between July 2009 and December 2010. The medical history and perioperative complications were compared between non-diabetic and diabetic groups. Length of stay, days awaiting surgery, and days of hospitalization after surgery were also analyzed. Ambulatory ability was compared at 1-year follow-up using the Chi-square test and Fisher's exact test. An independent Student's t-test was used to compare normally distributed continuous data.</p><p><b>RESULTS</b>Patients with diabetes were more likely than non-diabetic patients to develop cardiac perioperative complications (8.9% vs. 3.0%, P = 0.021), urinary tract infections (12.0% vs. 2.8%, P < 0.001), and gastrointestinal symptoms (15.0% vs. 6.8%, P = 0.003). No difference in perioperative complications was observed between the groups. Days awaiting surgery and length of hospital stay were both longer in the diabetic group ((8.0 ± 5.1) vs. (6.2 ± 3.7) days and (16.5 ± 3.8) vs. (13.3 ± 3.8) days, P < 0.001, respectively). Before the occurrence of fracture, patients with diabetes were less likely to be ambulatory outdoors (71.9% vs. 85.9%, P < 0.001) and had more restricted walking ability. After at least 1-year follow-up, similar proportions of patients in the non-diabetic and diabetic groups (16.1% and 15.9%, respectively), who were able to ambulate outdoors before the fracture, became housebound till the final follow-up.</p><p><b>CONCLUSIONS</b>Diabetics are at increased risk of specific complications and have a longer time to surgery and longer in-hospital stay, but generally have similar recovery to non-diabetics thereafter.</p>
Subject(s)
Aged , Aged, 80 and over , Female , Humans , Male , Middle Aged , Chi-Square Distribution , Diabetes Mellitus , Hip Fractures , Perioperative Period , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of E-selectin A561C (S128R) polymorphism on blood pressure.</p><p><b>METHODS</b>347 essential hypertensive patients (male 163 and female 184, age 46.08 +/- 12.49 y, and body mass index 26.13 +/- 3.14 kg/m(2)) and 315 normal controls (male 93 and female 222, age 42.21 +/- 14.67 y, and body mass index 25.66 +/- 3.19 kg/m(2)) were genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Phenotypic measurements included blood pressure, blood glucose, serum triglycerides, serum total, high-density lipoprotein (HDL) and low-density lipoprotein (LDL) cholesterol, serum uric acid, blood urea nitrogen, nitric oxide, endothelin, angiotensin I, and II and plasma aldosterone.</p><p><b>RESULTS</b>The frequencies of the AC and CC genotypes (6.92%) and C allele (4.76%) were significantly (P = 0.029 and 0.013) higher in hypertensive patients than normal controls (3.17% and 2.22%). The relative risk of hypertension associated with the C allele was 2.197 (95% CI: 1.164-4.144). Both diastolic blood pressure and mean arterial pressure were higher in C allele carriers than AA subjects (P = 0.049 and 0.024). Furthermore, C allele carriers, compared with AA subjects, had higher concentrations of blood glucose, and total and LDL cholesterol (P = 0.031, 0.046, and 0.003) and lower concentrations of nitric oxide (P = 0.036).</p><p><b>CONCLUSION</b>The E-selectin A561C (S128R) polymorphism might affect blood pressure in Chinese.</p>